While it’s probably most famous for its role in gene editing, CRISPR does more than just that: its ability to precisely cut and alter DNA could lead to new antibiotics, faster diagnosis tools, and more.
Hosted by: Hank Green.
Continue reading “4 Ways CRISPR Is More Than Just Gene Editing” »
Digital data storage is a growing need for our society and finding alternative solutions than those based on silicon or magnetic tapes is a challenge in the era of “big data.” The recent development of polymers that can store information at the molecular level has opened up new opportunities for ultrahigh density data storage, long-term archival, anticounterfeiting systems, and molecular cryptography. However, synthetic informational polymers are so far only deciphered by tandem mass spectrometry. In comparison, nanopore technology can be faster, cheaper, nondestructive and provide detection at the single-molecule level; moreover, it can be massively parallelized and miniaturized in portable devices. Here, we demonstrate the ability of engineered aerolysin nanopores to accurately read, with single-bit resolution, the digital information encoded in tailored informational polymers alone and in mixed samples, without compromising information density. These findings open promising possibilities to develop writing-reading technologies to process digital data using a biological-inspired platform.
DNA has evolved to store genetic information in living systems; therefore, it was naturally proposed to be similarly used as a support for data storage (1–3), given its high-information density and long-term storage with respect to existing technologies based on silicon and magnetic tapes. Alternatively, synthetic informational polymers have also been described (5–9) as a promising approach allowing digital storage. In these polymers, information is stored in a controlled monomer sequence, a strategy that is also used by nature in genetic material. In both cases, single-molecule data writing is achieved mainly by stepwise chemical synthesis (3, 10, 11), although enzymatic approaches have also been reported (12). While most of the progress in this area has been made with DNA, which was an obvious starting choice, the molecular structure of DNA is set by biological function, and therefore, there is little space for optimization and innovation.
Summary: Artificial intelligence technology redesigned a bacterial protein that helps researchers track serotonin in the brain in real-time.
Source: NIH
Serotonin is a neurochemical that plays a critical role in the way the brain controls our thoughts and feelings. For example, many antidepressants are designed to alter serotonin signals sent between neurons.
Continue reading “AI-Designed Serotonin Sensor May Help Scientists Study Sleep and Mental Health” »
Honorable Mentions
One more scientific brilliance this year is the use of light in neuroscience and tissue engineering. One study, for example, used lasers to directly print a human ear-like structure under the skin of mice, without a single surgical cut. Another used light to incept smell in mice, artificially programming an entirely new, never-seen-in-nature perception of a scent directly into their brains. Yet another study combined lasers with virtual reality to dissect how our brains process space and navigation, “mentally transporting” a mouse to a virtual location linked to a reward. To cap it off, scientists found a new way to use light to control the brain through the skull without surgery—though as of now, you’ll still need gene therapy. Given the implications of unauthorized “mind control,” that’s probably less of a bug and more of a feature.
We’re nearing the frustratingly slow, but sure, dying gasp of Covid-19. The pandemic defined 2020, but science kept hustling along. I can’t wait to share what might come in the next year with you—may it be revolutionary, potentially terrifying, utterly bizarre or oddly heart-warming.
Using CRISPR to alter the genetics of astrocytes in mice, researchers hope they’ve discovered how to regenerate neurons in patients with Parkinsons disease.
Using CRISPR/Cas9 gene editing tools, researchers introduced a common Parkinson’s disease mutation into stems cells of the marmoset monkey for a first time, paving the way toward a primate model of this disease.
As reported online Oct. 2, 2019, by Molecular Cell, a Harvard team was able to use the gene editing tool CRISPR to kill certain viruses, including the influenza virus, in a laboratory dish.
A new study has found that a novel T cell genetically engineered by University of Arizona Health Sciences researchers is able to target and attack pathogenic T cells that cause Type 1 diabetes, which could lead to new immunotherapy treatments.
The immune system fights bacteria, viruses and other pathogens by utilizing several types of T cells, all of which have receptors that are specific to particular antigens. On killer T cells, the receptor works in concert with three signaling modules and a coreceptor to destroy the infected cell. Michael Kuhns, Ph.D., an associate professor in the UArizona College of Medicine—Tucson Department of Immunobiology, copied the evolutionary design to engineer a five-module chimeric antigen receptor, or 5MCAR, T cell.
“The 5MCAR was an attempt to figure out if we could build something by biomimicry, using some of evolution’s natural pieces, and redirect T cells to do what we want them to do. We engineered a 5MCAR that would direct killer T cells to target autoimmune T cells that mediate Type 1 diabetes,” said Dr. Kuhns, who is member of the UArizona Cancer Center, BIO5 Institute and Arizona Center on Aging. “So now, a killer T cell will actually recognize another T cell. We flipped T cell-mediated immunity on its head.”
Researchers at EPFL have developed an approach to print tiny tissues that look and function almost like their full-sized counterpart. Measuring just a few centimeters across, the mini-tissues could allow scientists to study biological processes—and even test new treatment approaches—in ways that were previously not possible.
For years, mini versions of organs such as the brain, kidney and lung—known as “organoids”—have been grown from stem cells. Organoids promise to cut down on the need for animal testing and offer better models to study how human organs form and how that process goes awry in disease. However, conventional approaches to grow organoids result in stem cells assembling into micro-to millimeter-sized, hollow spheres. “That is non-physiological, because many organs, such as the intestine or the airway, are tube-shaped and much larger,” says Matthias Lütolf, a professor at EPFL’s Institute of Bioengineering, who led the study published today in Nature Materials.
Continue reading “Researchers develop new method to print tiny, functional organs” »
Researchers from the Francis Crick Institute (FCI) and University College London (UCL) have rebuilt a human thymus, an essential organ of the immune system, using human stem cells and a bioengineered scaffold. Their work is an important step towards being able to grow artificial thymi for use as transplants.
The thymus – located in the upper front part of the chest, behind the sternum – is a lymphoid organ where T cells mature. These play a vital role in the body’s immune system. If the thymus does not work properly or does not form during foetal development in the womb, it can result in severe immunodeficiency and other conditions where the body cannot fight infectious diseases or cancerous cells, or autoimmunity, where the immune system mistakenly attacks the patient’s own healthy tissue.
In their proof-of-concept study, published in Nature Communications, the scientists rebuilt thymi using stem cells taken from patients who had to have the organ removed during surgery. When transplanted into mice, the bioengineered thymi were able to support the development of mature and functional human T cells.
