Lifeboat Foundation: Safeguarding Humanity
Toggle light / dark theme

Blog

Category: bioengineering

Automated chloroplast screening platform speeds up crop trait development

Chloroplasts—the “light power plants” of plant cells—are increasingly the focus of synthetic biology. These organelles house the photosynthetic apparatus and host several metabolic pathways that are of great interest for engineering new traits. Gene insertion into chloroplasts is precise and carries a lower risk of transgene escape.

Despite this potential, chloroplast biotechnology remains in its infancy because standardized, scalable methods for rapid testing of diverse genetic parts have been missing. A research team from the Max Planck Institute for Terrestrial Microbiology in Marburg has now presented a micro‑algal platform that allows automated, fast, and large‑scale testing of chloroplast genetic modifications.

The study is published in the journal Nature Plants.

HUMAN Stem Cells Have Reversed Age In Monkey’s with ZERO Side Effects

Age Reversal in Primates has been achieved. We have it now.

Anti-aging gene therapy, stem cell rejuvenation, and FOXO3 longevity research take center stage in this episode of Longevity Science News with Emmett Short. This groundbreaking study out of Beijing shows that gene-edited human stem cells—specifically FOXO3-enhanced senescence-resistant mesenchymal progenitor cells (SRCs)—can reverse biological aging in elderly monkeys, restoring youthful brain structure, bone density, immune strength, and even ovarian function. By upgrading the FOXO3 longevity gene, scientists created stem cells that resist cellular senescence, DNA damage, and oxidative stress, effectively making the monkeys younger from the inside out. MRI scans revealed increased cortical thickness and improved memory-related connectivity, while biological age clocks showed a 3–5 year reversal across 54% of tissues—equivalent to 9–15 years of human rejuvenation. Emmett explains how these anti-aging stem cells, epigenetic resets, and exosome-based rejuvenation pathways could revolutionize regenerative medicine, longevity biotech, and future human trials. He also explores the costs, ethics, and long-term implications of turning back time at the cellular level. If you’re passionate about biohacking, gene editing, lifespan extension, or the future of anti-aging science, this is the video for you.

HUME BODY ANALYZER:
Use Code: LONGEVITY for up to 50% OFF
https://humehealth.com//discount/LONGEVITY?redirect=/pages/h…=LONGEVITY

Watch next: Artificial Blood: The SciFi Anti-Aging Tech That’s Now in Human Trials.
https://www.youtube.com/watch?v=3xz1lcGdQPc.

If you’re into the cutting edge of anti-aging science, subscribe and share with someone who wants to live longer, healthier, and stronger.

⏱️ Chapters:

Continue reading “HUMAN Stem Cells Have Reversed Age In Monkey’s with ZERO Side Effects” | >

Another protease, pepsin, cuts in the same general region of the antibody molecule as papain but on the carboxy-terminal side of the disulfide bonds (see Fig

3.3). This produces a fragment, the F(ab′)2 fragment, in which the two -binding arms of the antibody molecule remain linked. In this case the remaining part of the is cut into several small fragments. The F(ab′)2 fragment has exactly the same antigen-binding characteristics as the original antibody but is unable to interact with any effector molecule. It is thus of potential value in therapeutic applications of antibodies as well as in research into the functional role of the Fc portion.

Genetic engineering techniques also now permit the construction of many different -related molecules. One important type is a truncated Fab comprising only the of a linked by a stretch of synthetic peptide to a V domain of a . This is called , named from Fragment v ariable. Fv molecules may become valuable therapeutic agents because of their small size, which allows them to penetrate tissues readily. They can be coupled to protein toxins to yield immunotoxins with potential application, for example, in tumor therapy in the case of a Fv specific for a tumor (see Chapter 14).

Homo Invocator

We live immersed in a persistent illusion: the idea that consciousness arises from the brain like the flame from a candle. Contemporary science, constrained by the very instruments it creates, proclaims that the mind is merely the result of electrical impulses and chemical reactions — an epiphenomenon of flesh.

Yet a deeper look — one that doesn’t reject science but rather transcends it — reveals a more radical reality: we, living beings, are not the origin of consciousness, but rather its antenna.

We are hardware. Bodies shaped by millions of years of biological evolution, a complex architecture of atoms and molecules organized into a fractal of systems. But this hardware, no matter how sophisticated, is nothing more than a receptacle, a stage, an antenna. What truly moves, creates, and inspires does not reside here, within this tangible three-dimensional realm; it resides in an unlimited field, a divine matrix where everything already exists. Our mind, far from being an original creator, is a channel, a receiver, an interpreter.

The great question of our time — and perhaps of all human history — is this: how can we update the software running on this biological hardware without the hardware itself becoming obsolete? Herein lies the fundamental paradox: we can dream of enlightenment, wisdom, and transcendence, yet if the body does not keep pace — if the physical circuits cannot support the flow — the connection breaks, the signal distorts, and the promise of spiritual evolution stalls.

The human body, a product of Darwinian evolution’s slow dance, is both marvel and prison. Our eyes capture only a minuscule fraction of the electromagnetic spectrum; our ears are limited to a narrow range of frequencies; our brains filter out and discard 99% of the information surrounding us. Human hardware was optimized for survival — not for truth!

This is the first major limitation: if we are receivers of a greater reality, our apparatus is radically constrained. It’s like trying to capture a cosmic symphony with an old radio that only picks up static. We may glimpse flashes — a sudden intuition, an epiphany, a mystical experience — but the signal is almost always imperfect.

Thus, every spiritual tradition in human history — from shamans to mystery schools, from Buddhism to Christian mysticism — has sought ways to expand or “hack” this hardware: fasting, meditation, chanting, ecstatic dance, entheogens. These are, in fact, attempts to temporarily reconfigure the biological antenna to tune into higher frequencies. Yet we remain limited: the body deteriorates, falls ill, ages, and dies.

If the body is hardware, then the mind — or rather, the set of informational patterns running through it — is software: human software (and a limited one at that). This software isn’t born with us; it’s installed through culture, language, education, and experience. We grow up running inherited programs, archaic operating systems that dictate beliefs, prejudices, and identities.

Beneath this cultural software, however, lies a deeper code: access to an unlimited field of possibilities. This field — call it God, Source, Cosmic Consciousness, the Akashic Records, it doesn’t matter — contains everything: all ideas, all equations, all music, all works of art, all solutions to problems not yet conceived. We don’t invent anything; we merely download it.

Great geniuses throughout history — from Nikola Tesla to Mozart, from Leonardo da Vinci to Fernando Pessoa — have testified to this mystery: ideas “came” from outside, as if whispered by an external intelligence. Human software, then, is the interface between biological hardware and this divine ocean. But here lies the crucial question: what good is access to supreme software if the hardware lacks the capacity to run it?

An old computer might receive the latest operating system, but only if its minimum specifications allow it. Otherwise, it crashes, overheats, or freezes. The same happens to us: we may aspire to elevated states of consciousness, but without a prepared body, the system fails. That’s why so many mystical experiences lead to madness or physical collapse.

Thus, we arrive at the heart of the paradox. If the hardware doesn’t evolve, even the most advanced software download is useless. But if the software isn’t updated, the hardware remains a purposeless machine — a biological robot succumbing to entropy.

Contemporary society reflects this tension. On one hand, biotechnology, nanotechnology, and regenerative medicine promise to expand our hardware: stronger, more resilient, longer-lived bodies. On the other, the cultural software governing us remains archaic: nationalism, tribalism, dogma, consumerism. It’s like installing a spacecraft engine onto an ox-drawn cart.

At the opposite end of the spectrum, we find the spiritual movement, which insists on updating the software — through meditation, energy therapies, expanded states of consciousness — but often neglects the hardware. Weakened, neglected bodies, fed with toxins, become incapable of sustaining the frequency they aim to channel. The result is a fragile, disembodied spirituality — out of sync with matter.

Humanity’s challenge in the 21st century and beyond is not to choose between hardware and software, but to unify them. Living longer is meaningless if the mind remains trapped in limiting programs. Aspiring to enlightenment is futile if the body collapses under the intensity of that light.

It’s essential to emphasize: the power does not reside in us (though, truthfully, it does — if we so choose). This isn’t a doctrine of self-deification, but of radical humility. We are merely antennas. True power lies beyond the physical reality we know, in a plane where everything already exists — a divine, unlimited power from which Life itself emerges.

Our role is simple yet grand: to invoke. We don’t create from nothing; we reveal what already is. We don’t invent; we translate. A work of art, a mathematical formula, an act of compassion — all are downloads from a greater source.

Herein lies the beauty: this field is democratic. It belongs to no religion, no elite, no dogma. It’s available to everyone, always, at any moment. The only difference lies in the hardware’s capacity to receive it and the (human) software that interprets it.

But there are dangers. If the hardware is weak or the software corrupted, the divine signal arrives distorted. This is what we see in false prophets, tyrants, and fanatics: they receive fragments of the field, but their mental filters — laden with fear, ego, and the desire for power — twist the message. Instead of compassion, violence emerges; instead of unity, division; instead of wisdom, dogma.

Therefore, conscious evolution demands both purification of the software (clearing toxic beliefs and hate-based programming) and strengthening of the hardware (healthy bodies, resilient nervous systems). Only then can the divine frequency manifest clearly.

If we embrace this vision, humanity’s future will be neither purely biological nor purely spiritual — it will be the fusion of both. The humans of the future won’t merely be smarter or longer-lived; they’ll be more attuned. A Homo Invocator: the one who consciously invokes the divine field and translates it into matter, culture, science, and art.

The initial paradox remains: hardware without software is useless; software without hardware is impossible. But the resolution isn’t in choosing one over the other — it’s in integration. The future belongs to those who understand that we are antennas of a greater power, receivers of an infinite Source, and who accept the task of refining both body and mind to become pure channels of that reality.

If we succeed, perhaps one day we’ll look back and realize that humanity’s destiny was never to conquer Earth or colonize Mars — but to become a conscious vehicle for the divine within the physical world.

And on that day, we’ll understand that we are neither merely hardware nor merely software. We are the bridge.

Deep down, aren’t we just drifting objects after all?
The question is rhetorical, for I don’t believe any of us humans holds the answer.

__
Copyright © 2025, Henrique Jorge (ETER9)

Image by Gerd Altmann from Pixabay

[ This article was originally published in Portuguese in Link to Leaders at: https://linktoleaders.com/o-ser-como-interface-henrique-jorge-eter9/]

Scientists just made gene editing far more powerful

Scientists at The University of Texas at Austin have developed a revolutionary gene-editing method using bacterial retrons that can correct multiple disease-causing mutations at once. Unlike traditional tools limited to one or two mutations, this retron-based system replaces large defective DNA regions, dramatically improving efficiency and inclusivity for patients with complex disorders like cystic fibrosis.

The Rise of Mechanobiology for Advanced Cell Engineering and Manufacturing

The rise of cell-based therapies, regenerative medicine, and synthetic biology, has created an urgent need for efficient cell engineering, which involves the manipulation of cells for specific purposes. This demand is driven by breakthroughs in cell manufacturing, from fundamental research to clinical therapies. These innovations have come with a deeper understanding of developmental biology, continued optimization of mechanobiological processes and platforms, and the deployment of advanced biotechnological approaches. Induced pluripotent stem cells and immunotherapies like chimeric antigen receptor T cells enable personalized, scalable treatments for regenerative medicine and diseases beyond oncology. But continued development of cell manufacturing and its concomitant clinical advances is hindered by limitations in the production, efficiency, safety, regulation, cost-effectiveness, and scalability of current manufacturing routes. Here, recent developments are examined in cell engineering, with particular emphasis on mechanical aspects, including biomaterial design, the use of mechanical confinement, and the application of micro-and nanotechnologies in the efficient production of enhanced cells. Emerging approaches are described along each of these avenues based on state-of-the-art fundamental mechanobiology. It is called on the field to consider mechanical cues, often overlooked in cell manufacturing, as key tools to augment or, at times, even to replace the use of traditional soluble factors.

Current manufacturing workflows for CAR-based immunotherapies, particularly CAR T, and the emerging CAR NK and CAR macrophage platforms, generally involve four key stages: (i) isolation of primary immune cells or their precursors, (ii) cell activation or differentiation, (iii) genetic modification with CAR constructs, most often via viral vectors or electroporation (EP), and (iv) expansion or preparation for reinfusion. Among these, transfection remains the most critical and technically challenging step, directly influencing the functionality, safety, and scalability of the final product.

In clinical-scale production, EP remains the most widely used non-viral method for gene delivery into immune cells, yet it is increasingly recognized as suboptimal, particularly when delivering large or complex CAR constructs. It suffers from inefficient nuclear delivery, high cell toxicity, and poor functional yields of viable, potent CAR-expressing cells.[ 113 ] These limitations are further exacerbated in more fragile or less permissive cell types, such as NK cells and macrophages, which show lower transfection efficiencies and greater sensitivity to electroporation-induced stress.[ 114 ] Viral vectors, while still dominant in clinical manufacturing, present their own challenges: they are constrained by limited cargo capacity, are costly to produce at scale, and raise regulatory and safety concerns, especially when applied to emerging CAR-NK and CAR macrophage therapies that require flexible, transient, or multiplexed genetic programs.[ 115 ]

In contrast to immune-cell engineering, stem cell-based approaches present a different set of challenges and engineering requirements. While immune cells are genetically modified to enhance cytotoxicity[ 116 ] and specificity or to mitigate excessive T-cell activation,[ 117 ] stem cells must be engineered to control self-renewal, lineage commitment, and functional integration, often requiring precise, non-integrative delivery of genetic or epigenetic modulators (e.g., mRNA, episomal vectors) to maintain cellular identity and safety.[ 118 ] Stem cells hold exceptional therapeutic promise due to their capacity for self-renewal and differentiation into specialized cell types, supporting applications in personalized disease modeling, tissue repair, and organ regeneration.[ 119 ] However, engineering stem cells in a safe, efficient, and clinically relevant manner remains a major challenge. Conventional delivery methods, such as viral vectors and EP, can compromise genomic integrity,[ 120 ] reduce viability,[ 118 ] and induce epigenetic instability,[ 121 ] limiting their translational potential.

Chemical networks can mimic nervous systems to power movement in soft materials

What if a soft material could move on its own, guided not by electronics or motors, but by the kind of rudimentary chemical signaling that powers the simplest organisms? Researchers at the University of Pittsburgh Swanson School of Engineering have modeled just that—a synthetic system that on its own directly transforms chemical reactions into mechanical motion, without the need for the complex biochemical machinery present in our bodies.

Just like jellyfish, some of the simplest organisms do not have a centralized brain or . Instead, they have a “nerve net” which consists of dispersed nerve cells that are interconnected by active junctions, which emit and receive . Even without a central “processor,” the chemical signals spontaneously travel through the net and trigger the autonomous motion needed for organisms’ survival.

In a study published in PNAS Nexus, Oleg E. Shklyaev, research assistant, and Anna C. Balazs, Distinguished Professor of Chemical and Petroleum Engineering and the John A. Swanson Chair of Engineering, have developed computer simulations to design a with a “nerve net” that links chemical and mechanical networks in a way that mimics how the earliest and simplest living systems coordinate motion.

MIT’s new precision gene editing tool could transform medicine

MIT scientists have found a way to make gene editing far safer and more accurate — a breakthrough that could reshape how we treat hundreds of genetic diseases. By fine-tuning the tiny molecular “tools” that rewrite DNA, they’ve created a new system that makes 60 times fewer mistakes than before.

Lab-grown brains with all major cell types support next-generation therapy research

A new 3D human brain tissue platform developed by MIT researchers is the first to integrate all major brain cell types, including neurons, glial cells and the vasculature into a single culture. Grown from individual donors’ induced pluripotent stem cells, these models—dubbed Multicellular Integrated Brains (miBrains)—replicate key features and functions of human brain tissue, are readily customizable through gene editing, and can be produced in quantities that support large-scale research.

Although each unit is smaller than a dime, miBrains may be worth a great deal to researchers and drug developers who need more complex living lab models to better understand brain biology and treat diseases.

“The miBrain is the only in vitro system that contains all six major cell types that are present in the human brain,” said Li-Huei Tsai, Picower Professor, director of The Picower Professor of Learning and Memory, and senior author of the study describing miBrains, published in the Proceedings of the National Academy of Sciences.

/* */