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Category: bioengineering – Page 135

A little bit of norovirus—the highly infectious microbe that causes about 20 million cases of food poisoning in the United States each year—goes a long way. Just 10 particles of the virus can cause illness in humans. A team of University of Arizona researchers has created a simple, portable and inexpensive method for detecting extremely low levels of norovirus.

Jeong-Yeol Yoon, a researcher in the Department of Biomedical Engineering; Soo Chung, a biosystems engineering doctoral student who works in Yoon’s Biosensors Lab; and Kelly A. Reynolds, Chair of the Department of Community, Environment and Policy in the Mel & Enid Zuckerman College of Public Health, led the project. The team published their results in ACS Omega, the official journal of the American Chemical Society, and Yoon is presenting the research at the ACS Fall 2019 National Meeting & Exposition in San Diego this week.

“Advances in rapid monitoring of human viruses in water are essential for protecting public health,” Reynolds said. “This rapid, low-cost water quality monitoring technology could be a transformational tool for reducing both local and global disease burdens.”

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Stem cell biologist Hiromitsu Nakauchi has been waiting for this moment for more than a decade.

After years of planning, the persistent researcher has at last received approval from a government willing to pursue one of the most controversial scientific studies there is: human-animal embryo experiments.

While many countries around the world have restricted, defunded or outright banned these ethically-fraught practices, Japan has now officially lifted the lid on this proverbial Pandora’s box. Earlier this year, the country made it legal to not only transplant hybrid embryos into surrogate animals, but also to bring them to term.

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New York, NY—August 12, 2019—A novel neck brace, which supports the neck during its natural motion, was designed by Columbia engineers. This is the first device shown to dramatically assist patients suffering from Amyotrophic Lateral Sclerosis (ALS) in holding their heads and actively supporting them during range of motion. This advance would result in improved quality of life for patients, not only in improving eye contact during conversation, but also in facilitating the use of eyes as a joystick to control movements on a computer, much as scientist Stephen Hawkins famously did.

A team of engineers and neurologists led by Sunil Agrawal, professor of mechanical engineering and of rehabilitation and regenerative medicine, designed a comfortable and wearable robotic neck brace that incorporates both sensors and actuators to adjust the head posture, restoring roughly 70% of the active range of motion of the human head. Using simultaneous measurement of the motion with sensors on the neck brace and surface electromyography (EMG) of the neck muscles, it also becomes a new diagnostic tool for impaired motion of the head-neck. Their pilot study was published August 7 in the Annals of Clinical and Translational Neurology.

The brace also shows promise for clinical use beyond ALS, according to Agrawal, who directs the Robotics and Rehabilitation (ROAR) Laborator y. “The brace would also be useful to modulate rehabilitation for those who have suffered whiplash neck injuries from car accidents or have from poor neck control because of neurological diseases such as cerebral palsy,” he said.

Continue reading “Robotic Neck Brace Dramatically Improves Functions of ALS Patients” | >

Gene editing can turn living cells into minicomputers that can read, write and perform complex calculations. The technology could track what happens inside the body over time.

DNA computers have been around since the 1990s, when researchers created DNA molecules able to perform basic mathematical functions. Instead of storing information as 0s and 1s like digital computers do, these computers store information in the molecules A, C, G and T that make up DNA.

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An international team of researchers with partial support from the National Institute of Biomedical Imaging and Bioengineering (NIBIB) developed a new MRI technique that can capture an image of a brain thinking by measuring changes in tissue stiffness. The results show that brain function can be tracked on a time scale of 100 milliseconds – 60 times faster than previous methods. The technique could shed new light on altered neuronal activity in brain diseases.

The human brain responds almost immediately to stimuli, but non-invasive imaging techniques haven’t been able to keep pace with the brain. Currently, several non-invasive brain imaging methods measure brain function, but they all have limitations. Most commonly, clinicians and researchers use functional magnetic resonance imaging (fMRI) to measure brain activity via fluctuations in blood oxygen levels. However, a lot of vital brain activity information is lost using fMRI because blood oxygen levels take about six seconds to respond to a stimulus.

Since the mid-1990s, researchers have been able to generate maps of tissue stiffness using an MRI scanner, with a non-invasive technique called magnetic resonance elastography (MRE). Tissue stiffness can’t be measured directly, so instead researchers use MRE to measure the speed at which mechanical vibrations travel through tissue. Vibrations move faster through stiffer tissues, while vibrations travel through softer tissue more slowly; therefore, tissue stiffness can be determined. MRE is most commonly used to detect the hardening of liver tissue but has more recently been applied to other tissues like the brain.

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Circa 2017

Efficient intracellular delivery of biologically active macromolecules has been a challenging but important process for manipulating live cells for research and therapeutic purposes. There have been limited transfection techniques that can deliver multiple types of active molecules simultaneously into single-cells as well as different types of molecules into physically connected individual neighboring cells separately with high precision and low cytotoxicity. Here, a high frequency ultrasound-based remote intracellular delivery technique capable of delivery of multiple DNA plasmids, messenger RNAs, and recombinant proteins is developed to allow high spatiotemporal visualization and analysis of gene and protein expressions as well as single-cell gene editing using clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein-9 nuclease (Cas9), a method called acoustic-transfection. Acoustic-transfection has advantages over typical sonoporation because acoustic-transfection utilizing ultra-high frequency ultrasound over 150 MHz can directly deliver gene and proteins into cytoplasm without microbubbles, which enables controlled and local intracellular delivery to acoustic-transfection technique. Acoustic-transfection was further demonstrated to deliver CRISPR-Cas9 systems to successfully modify and reprogram the genome of single live cells, providing the evidence of the acoustic-transfection technique for precise genome editing using CRISPR-Cas9.

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